WEST CHESTER TOWNSHIP — The unknown substance found in a vial at a Frisch's restaurant here has tested “negative for biologicals,” Patricia Burg, administrative director of the Butler County Health Department, said Friday.

        Ms. Burg said the Ohio Department of Health's Columbus office did the testing and the FBI informed her of the result Friday morning.

        Further testing, which would be needed to identify the substance, probably will not be conducted, she said.

        An employee of the Frisch's on Cincinnati-Dayton Road reportedly tasted the substance on Monday night, then developed a rash and red eyes — a report that Ms. Burg said now appears to be false.

        On Tuesday, Frisch's employees called police because they were suspicious that a couple who had left the small, one-ounce vial had returned to retrieve it. The report led to the restaurant's daylong closure; more than two dozen people were quarantined inside for several hours.

        Hazardous-materials personnel, wearing protective gear, quadruple-bagged the substance as a precaution.

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